IBWS: IST Bioinformatics Web Services
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IST Bioinformatics Web Services: CABRI: Human and animal cell lines

Services
Currently, the following Web Services are available:
getCellLineIdsByName - getCellLineIdsByProperty - getCellLinesById


getCellLineIdsByName: Get cell lines ID searching by name (wsdl)

Inputs:
<libs>
acronym(s) of searched CABRI catalogues (MANDATORY).
When providing more acronyms, they MUST be separated by the '_SP_' string. E.g.: "ICLC_SP_DSMZ_MUTZ" instead of "ICLC DSMZ_MUTZ"
No wild character (*,?,%) is allowed.

<name>
name of searched cell lines (MANDATORY).
Punctuation and spaces must be removed from the name. E.g.: "A704" instead of "A-704"
The wild character '*' is allowed.

Output:
a list of unique IDs preceeded by the acronym of the related catalogue (<catalogue_acronym>:<unique_id>)

Example:

Inputs: <libs>: ICLC_SP_DSMZ_MUTZ ; <name>: hela*
Output:
   DSMZ_MUTZ:ACC 57
   DSMZ_MUTZ:ACC 161
   ICLC:ICLC HTL95023
   ICLC:ICLC HTL95015
   ICLC:ICLC HTL95014
   ICLC:ICLC HTL95016
   ICLC:ICLC HTL95020
Test this Web Services
Libs:
Name:

getCellLineIdsByProperty: Get cell lines ID searching by properties (all text) (wsdl)

Inputs:
<libs>
acronym(s) of searched CABRI catalogues (MANDATORY).
When providing more acronyms, they MUST be separated by the '_SP_' string. E.g.: "ICLC_SP_DSMZ_MUTZ" instead of "ICLC DSMZ_MUTZ"
No wild character (*,?,%) is allowed.

<text>
query terms to be searched for (MANDATORY).
The wild character '*' is allowed.

Output:
a list of unique IDs preceeded by the acronym of the related catalogue (<catalogue_acronym>:<unique_id>)

Example:

Input: <libs>: ICLC_SP_DSMZ_MUTZ ; <text>: rhabdomyosarcoma
Output:
   DSMZ_MUTZ:ACC 250
   DSMZ_MUTZ:ACC 493
   DSMZ_MUTZ:ACC 491
   DSMZ_MUTZ:ACC 489
   DSMZ_MUTZ:ACC 592
   DSMZ_MUTZ:ACC 263
   ICLC:ICLC HTL99002
   ICLC:ICLC HTL97021
Test this Web Services
Libs:
Text:

getCellLinesById: Get cell lines details searching by ID (wsdl)

Inputs:
<libs>
acronym(s) of searched CABRI catalogue (MANDATORY).
No wild character (*,?,%) is allowed.

<ID>
unique ID for a record in the specified catalogue (MANDATORY).
Space MUST be substituted by the '_SP_' string. E.g.: "ICLC_SP_HTL03003" instead of "ICLC HTL03003"
No wild character (*,?,%) is allowed.

Output:
the full record of the specified ID in the searched catalogue

Example:

Inputs: <libs>: ICLC ; <ID>: ICLC_SP_HTL03003
Output:
  Accession_number ICLC HTL03003
  Cell_line_name 293
  Brief_description Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
  Description Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
  Depositor Obtained from ECACC
  Bibliographic_reference J Gen Virol 1977;36:59-74 [PMID: 886304]
  Morphology epithelial-like, grown as monolayer
  Culture_conditions continuous culture; DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids;
    Split cultures 1:3-1:6 using trypsin/EDTA; 37?C, 5% CO2. The viability may be low after thawing and
    the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule
    and thawed with 20% FBS
  Viruses Search for viruses was not performed
  Release_conditions Cell line available for distribution. For non-commercial investigative use only
  Hazard Handle as Biosafety Level 2 containment
  Freezing_medium 90% FBS + 10% DMSO
  Sterility mycoplasma negative, HOECHST and PCR
  Validation_assays Multiplex PCR: tested against human, rat, mouse primers.
    Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
  Further_bibliography Virology 1977;77:319-329 [PMID: 841862]
Test this Web Services
Libs:
ID:



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