IBWS: IST Bioinformatics Web Services | |||||||
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IST Bioinformatics Web Services: SWS: querySWS
querySWS: Query a specified library and return entries related to queried terms
(wsdl)
Examples: querySWS -lib ICLC -query hela -output_fields "cell_line_name hazard" Inputs: <db_acronym>: ICLC ; <terms>: hela Output: Accession_number ICLC HTL95023 Cell_line_name HeLa Brief_description Species: human, Black; Tissue: cervix; Tumor: carcinoma, epitheloid Description Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Depositor Obtained from ECACC Bibliographic_reference Cancer Res 1952;12:264 Morphology epithelial-like, grown as monolayer Culture_conditions continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3x104 cells/cm2; 37C, 5% CO2 Viruses Search for viruses was not performed Properties antitumour testing; transformation; tumorigenicity; cytotoxicity; bacterial invasiveness; virology; Susceptible to: adenovirus 3, measles, poliovirus 1, echovirus, vaccinia, arbovirus, respiratory syncytial virus, reovirus 3, rhinovirus, Coxsackie Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard - Species_validation Validated by isoenzymes: confirmed as human with AST, G6PD, MD Tumorigenicity tumorigenic in nude mice Karyology aneuploid Freezing_medium Culture medium + 50% FBS + 10% DMSO Sterility mycoplasma negative, HOECHST and PCR Further_bibliography J Exp Med 1953;97:695 Obstet Gynecol 1971;38:945-949 [PMID: 4942173] Inputs: <db_acronym>: ICLC ; <terms>: hela ; <output_fields>: cell_line_name Output: Cell_line_name HeLa Cell_line_name HeLa 382 Cell_line_name HeLa 422 Cell_line_name HeLa 432 Cell_line_name HeLa S3 Cell_line_name KB |
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Webmaster: Paolo Romano; Last update: January 28, 2020. |