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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). The addition of Bovine Insulin has been reported to be useful where there has been slow growth of cells. Split sub-confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. NB: During routine subculture the cells should always be subcultured before they achieve confluence.
  Passages: 20; Karyotype: Hypertetraploid, modal No 96
  Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.
  Research council deposit: No; Price Code: A;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: HTB 37, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
  
• Biol Cell 1983;47:323-330
  
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
  
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
  
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
  

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
  MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4x104 cells/cm2. After thawing, cells might grow slowly. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR - mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
  Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 16,18; FGA: 19; TH01: 6; D18S51: 12; D21S11: 30; D8S1179: 12) * isoenzymes (confirmed as human with MD, NP)
  

Availability in cell line catalogues: ECACC: 86010202, DSMZ: ACC 169, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
• Biol Cell 1983;47:323-330
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  MEM (EBSS) + 15% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 18 , mycoplasma negative, culture
  Validated by: isoenzymes (confirmed as human with NP, G6PD, MPI, PEPB)
  

Availability in cell line catalogues: ATCC: HTB-37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023

Bibliographic references:

• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Pharmacia Upjhon (MIECC, Nerviano (MI))
  MEM (EBSS) + 20% FBS + 1% non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3
  

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
• Biol Cell 1983;47:323-330
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
  MEM + 15% FBS + Antibiotics + 1% non-essential amino acids + 20mM Tricine + 1mM Na pyruvate; Freezing medium: Culture medium + 20% FBS + 10% Glycerol; 37C, 5% CO2
  Split 1:4 weekly.
  Validated by: biochemical assays * cellular assays
  

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
• Biol Cell 1983;47:323-330
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Istituto Superiore di Sanità (RMSTC, Roma)
  DMEM + 5% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: DMEM + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-9 , mycoplasma negative
  

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
• Biol Cell 1983;47:323-330
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080

CACO-2 (human, Caucasian, colon, adenocarcinoma)

Available in the following LABORATORY:

• Russian Academy of Sciences (SPBIC, St. Petersburg)
  mycoplasma negative
  

Availability in cell line catalogues: ATCC: HTB 37, ECACC: 86010202, DSMZ: ACC 169, ICLC: HTL97023, IZSBS: BS TCL 87

Bibliographic references:

• Biochimie 1986;68:1035-1040 - PMID: 3096381
• Biol Cell 1983;47:323-330
• Exp Cell Res 1992;200:97-104 - PMID: 1563496
• J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
• J Natl Cancer Inst 1977;59:221-226 - PMID: 327080