SK-HEP-1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity

Available in the following LABORATORY:

Dip. di Scienze Biomediche e Biotecnologie (BSUPG, Brescia)
MEM + 10% FBS + 1% non-essential amino acids; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-5
Isolated from ascitic fluid

Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141, ICLC: HTL10001

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma

DSMZ catalog code: ACC 141
Morphology: epithelial-like cells growing as monolayer
Description: human liver adenocarcinoma established from the ascites of a 52-year-old Caucasian man in 1971 confirmed as human with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80% RPMI 1640 + 20% FBS split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast; seed out at ca. 3-4 x 106 cells/80 cm 2 at 37C with 5% CO2 about 15-18 x 106 cells/175 cm 2; doubling time of ca. 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotriploid karyotype with 9% polyploidy; 62/63(54-64)<3n>X/XX, -Y, -3, -4, +6, +7, -9, -13, -13, -13, -14, -15, -16, -16, +17, -18, +19, +20, -21, +22, +mar, der(1)t(1;?)(q21;?), t(2;20)(p11;p11), del(6)(q25), der(14)t(14;?) * cytogenetics 2 (i(14q), der(19)t(19;?)(p13;?); sidelines carry t(14q15q) for i(14q) and der(12)t(12;?)(p12.3;?)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin++, desmin-, endothel-, GFAP-, neurofilament-, vimentin++) * isoenzymes (confirmed as human with IEF of AST, MDH, PEP B) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, ICLC: HTL10001

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

ICLC catalog code: HTL10001
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
DEPOSITOR: A M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2 , mycoplasma contamination eliminated with plasmocin; negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)

Availability in cell line catalogues: ECACC: 91091816, DSMZ: ACC 141

SK-HEP-1 (human, Caucasian, liver, adenocarcinoma)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 52 years old) Karyology: hyperdiploid to hypotriploid
TISSUE/ORGAN: liver
TUMOR: adenocarcinoma
tumorigenic in nude mice
PROPERTIES: bFGF prod.; tumorigenicity

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative
Tissue: liver, ascites fluid.

Availability in cell line catalogues: ATCC: HTB 52, ECACC: 91091816, DSMZ: ACC 141, ICLC: HTL10001

Bibliographic references:

Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - PMID: 327080
Natl Cancer Inst Monogr 1978;49:5-19 - PMID: 571047

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105