HT-29's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

ICLC catalog code: HTL99026
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 44 years old)
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma, grade II
tumorigenic
DEPOSITOR: Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production. HLA: A1, A3; B12, B17; Cw5.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
McCoy's 5A + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:5 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 17,19; FGA: 20,22; TH01: 6,9; D18S51: 13,9.2; D21S11: 29,30; D8S1179: 10,16)

Availability in cell line catalogues: IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Human tumor cells in vitro. Fogh J. ed., Plenum Press, New York, 1975; pp. 115-159

HT-29 (human, Caucasian, colon, adenocarcinoma)

IZSBS catalog code: BS TCL 132
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: human, Caucasian (female, 44-year-old) Karyology: 2n = 46 in 50 cells
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma
tumorigenic in steroid-treated hamsters
DEPOSITOR: obtained from ATCC
Distribution is subjected to authorization by the assignee.
Patent train
PROPERTIES: producing carcinoembryonic antigen; releasing secretory component of IgA

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
McCoy's 5A + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 130 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD)

Availability in cell line catalogues: ATCC: HTB-38, ICLC: HTL99026, DSMZ: ACC 299

Bibliographic references:

Human Tumor Cells In Vitro 1975;115-159, J. Fogh (ed.) Plenum Press, New York

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (female, 44 years old) Karyology: hypertriploid
TISSUE/ORGAN: colon
TUMOR: adenocarcinoma, grade II
tumorigenic in nude mice
Susceptibility to viruses: vesicular stomatitis
PROPERTIES: specialized product secr.; IgA prod.; tumor markers; CEA prod.; antitumour testing; tumorigenicity; differentiation; pharmacodynamics; cloning; cytotoxicity; cell biology; antineoplastic agents sensibility; virology

Available in the following LABORATORY:

Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
McCoy's 5A + 10% FBS; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-6 Passage: 125 , mycoplasma negative
Validated by: isoenzymes (Me-2,1; PGM3,1-2; PGM1,1-2; ES D,1; AK1,1; GLO-1,1-2; G6PD,B)

Availability in cell line catalogues: ATCC: HTB 38, ICLC: HTL99026, IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Cancer Res 1988;48:6193-6200 - PMID: 3167866
Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

Available in the following LABORATORY:

Istituto Ricerche Farmacologiche Mario Negri (MIILC, Milano)
MEM (HBSS) + 10% FBS + 1% non-essential amino acids + 2x Vitamins + NaHCO3; Freezing medium: Culture medium + 30% FBS + 15% DMSO; 37C, 5% CO2, split 1-20 Passage: 55 , mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 38, ICLC: HTL99026, IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Cancer Res 1988;48:6193-6200 - PMID: 3167866
Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
Ham's F12 + 10% FBS + 1% Vitamins; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: HTB 38, ICLC: HTL99026, IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Cancer Res 1988;48:6193-6200 - PMID: 3167866
Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)
mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 38, ICLC: HTL99026, IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Cancer Res 1988;48:6193-6200 - PMID: 3167866
Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

HT-29 (human, Caucasian, colon, adenocarcinoma, grade II)

Available in the following LABORATORY:

Istituto Regina Elena (RMICS, Roma)
RPMI 1640 + 10% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 30% FBS + 15% DMSO; 37C, 5% CO2 Passage: 100
Seed 1 x 106 cells/T25.

Availability in cell line catalogues: ATCC: HTB 38, ICLC: HTL99026, IZSBS: BS TCL 132, DSMZ: ACC 299

Bibliographic references:

Cancer Res 1988;48:6193-6200 - PMID: 3167866
Human Tumor Cells in Vitro (Plenum Press N Y) 1975;115-159
J Natl Cancer Inst 1977;58:209-214 - PMID: 833871

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105