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ICLC catalog code: ATL07002
Continuous culture, grown as monolayer
SPECIES: mouse, CD-1 transgenic (female)
TISSUE/ORGAN: mammary gland
TRANSFORMED BY: MMTVneu oncogene Back To Oncogene Agents' WebPage
DEPOSITOR: Dr M.G. Sacco, Human Genome Dept, ITB-CNR, Milan, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
William's medium + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 10% FBS + 10% DMSO; split confluent cultures 1:2-1:4; 37C, 5% CO2 Passage: 19, mycoplasma negative, HOECHST and PCR
fluorescent cells. Cells also grow well with the heat inactivated serum
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
- Cell Prolif. 2006 Dec;39(6):611-22. - PMID: 17109643
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.