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DSMZ catalog code: ACC 204
Morphology: adherent epitheloid cells growing in monolayers
Description: human pancreas adenocarcinoma established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman (sister cell line is PA-TU-8988T, DSM ACC 162) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. P. Elsaesser, University of Marburg, Marburg, Germany
Available in the following LABORATORY:
German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 5% FBS + 5% horse serum + 2 mM L-glutamine split confluent culture 1:10 once a week using trypsin/EDTA; seed out at ca. 4 x 106 cells/80 cm 2 at 37C with 5-10% CO2 cell harvest of about 30 x 106 cells/80 cm 2;
doubling time of about 40-60 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (human near triploid karyotype with 6% polyploidy; 71(67- 77)<3n>XXX, +3, -4, +7, -8, +9, +11, +11, -15, -17, -18, -18, -19, -21, - 22, +6mar, del(1)(p21), del(3)(p11p22), del(6)(q26), del(9)(p12), dup(11)(p14->pter), del(12)(p13) a) * cytogenetics 2 ((p12)) * DNA fingerprinting (unique profile with (gtg)5 and pYNH24 probes, but identical with PA-TU-8988T) * immunological analysis (cytokeratin + (100%), desmin -, endothel -, GFAP -, neurofilament -, vimentin-) * isoenzymes (confirmed as human with IEF of AST, MDH)
- Virchows Arch B Cell Pathol 1992;61:295-306
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.