3T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

3T3 (mouse, Swiss albino, fibroblast)

SPECIES: mouse, Swiss albino
TISSUE/ORGAN: fibroblast

DSMZ catalog code: ACC 173
Morphology: fibroblasts growing adherently as monolayer with contact inhibition
Description: Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition (= ATCC CCL 92) confirmed as mouse with IEF of AST, LDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of about 5-8 x 106 cells/175 cm 2; doubling time of ca. 40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at 1 x 106 cells/ampoule; mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy; 68(58-73)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, LDH, PEP B)

Availability in cell line catalogues: ICLC: ATL95002, IZSBS: BS CL 82

Bibliographic references:

J Cell Biol 1963;17:299-313
Virology 1965;27:179-185 - PMID: 4284655

3T3 (mouse, Swiss albino, embryo)

ICLC catalog code: ATL95002
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, Swiss albino
TISSUE/ORGAN: embryo
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Contact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with G6PD, MD, NP)

Availability in cell line catalogues: DSMZ: ACC 173, IZSBS: BS CL 82

Bibliographic references:

J Cell Biol 1963;17:299-313 (reference paper)
Virology 1965;27:179-185 - PMID: 4284655

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105