3T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

3T3 (mouse, Swiss albino, fibroblast)

SPECIES: mouse, Swiss albino
TISSUE/ORGAN: fibroblast

DSMZ catalog code: ACC 173
Morphology: fibroblasts growing adherently as monolayer with contact inhibition
Description: Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition (= ATCC CCL 92) confirmed as mouse with IEF of AST, LDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm 2 at 37C with 5% CO2 cell harvest of about 5-8 x 106 cells/175 cm 2; doubling time of ca. 40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at 1 x 106 cells/ampoule; mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy; 68(58-73)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, LDH, PEP B)

Availability in cell line catalogues: ICLC: ATL95002, IZSBS: BS CL 82

Bibliographic references:

J Cell Biol 1963;17:299-313
Virology 1965;27:179-185 - PMID: 4284655

3T3 (mouse, Swiss albino, embryo)

IZSBS catalog code: BS CL 82
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, Swiss albino Karyology: 2n = 40 in 48 cells
TISSUE/ORGAN: embryo
non tumorigenic
Susceptibility to viruses: herpes simplex * polyoma virus * Sendai * SV40 * vesic. stomatitis (Indiana)
PROPERTIES: Conctat inhibited. The cell line is readily transformed by two oncogenic viruses, polyoma and SV40.

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 116 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, PEPB, AST, LD)

Availability in cell line catalogues: ATCC: CCL 92, DSMZ: ACC 173, ICLC: ATL95002

Bibliographic references:

Arch Virol 1984;80:47 - PMID: 6200094

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105