3T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

3T3 (mouse, Swiss albino, embryo)

ICLC catalog code: ATL95002
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, Swiss albino
TISSUE/ORGAN: embryo
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Contact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with G6PD, MD, NP)

Availability in cell line catalogues: DSMZ: ACC 173, IZSBS: BS CL 82

Bibliographic references:

J Cell Biol 1963;17:299-313 (reference paper)
Virology 1965;27:179-185 - PMID: 4284655

3T3 (mouse, Swiss albino, embryo)

IZSBS catalog code: BS CL 82
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mouse, Swiss albino Karyology: 2n = 40 in 48 cells
TISSUE/ORGAN: embryo
non tumorigenic
Susceptibility to viruses: herpes simplex * polyoma virus * Sendai * SV40 * vesic. stomatitis (Indiana)
PROPERTIES: Conctat inhibited. The cell line is readily transformed by two oncogenic viruses, polyoma and SV40.

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 116 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as mouse with NP, G6PD, PEPB, AST, LD)

Availability in cell line catalogues: ATCC: CCL 92, DSMZ: ACC 173, ICLC: ATL95002

Bibliographic references:

Arch Virol 1984;80:47 - PMID: 6200094

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105