Version 4.0.201701 Home - Cell Index - Search - Web Sites Species - Tissues - Tumors - Pathologies - Tr. Agents Collections - Laboratories - Catalogues
For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SV-T2 (mouse, BALB/c, embryo)

Available in the following LABORATORY:

• Istituto di Cancerologia, Universita' degli Studi (BOUCN, Bologna)
  DMEM + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 8-16 Passage: 160 , mycoplasma negative, HOECHST
  

Availability in cell line catalogues: ATCC: CCL 163.1, IZSBS: BS CL106, ECACC: 90030801

Bibliographic references:

• J Cell Physiol 1968;72:141-148 - PMID: 4301006
• Science 1968;162:1024-1026 - PMID: 4301647

SV-T2 (mouse, BALB/c, embryo)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Fetal Bovine Serum (FBS)). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1x1,000 to 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Passages: 155; Karyotype: 2n = 40, modal No 41
  Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts. The cells should be handled under laboratory containment level 2.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 163.1, IZSBS: BS CL106

Bibliographic references:

• J Cell Physiol 1968;72:141-148 - PMID: 4301006
  
• Science 1968;162:1024-1026 - PMID: 4301647