PA317's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

PA317 (mouse, NIH Swiss, embryo)

ICLC catalog code: HTL06006
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo
Original line: TK- NIH/3T3 cells
Transfected by: (pPAM3) and HSV thymidine kinase
DEPOSITOR: Dr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Packaging line for retroviruses

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 5% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2 Passage: 3 , mycoplasma negative, DAPI and HOECHST
Handle under Biosafety Level 2 containment
amphotropic packaging cell line

Availability in cell line catalogues: ECACC: 89032007

Bibliographic references:

J Biol Chem 1996;271: 13968-13975 - PMID: 8662928
J Virol 1997;71: 345-353 - PMID: 8985356
Mol Cell Biol 1986; 6: 2895-2902 - PMID: 3785217
N Engl J Med 1990;323: 570-578 - PMID: 2381442

PA317 (mouse, NIH Swiss, fibroblast)

Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: fibroblast
Original line: TK- NIH/3T3
Transfected by: retrovirus packaging construct DNA (p PAM3) and herpes TK gene
PROPERTIES: transfection; virology; genetical transformation

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 9078, ECACC: 89032007, ICLC: HTL06006

Bibliographic references:

Mol Cell Biol 1986;6:2895-2902 - PMID: 3537716
N Engl J Med 1990;232:570-578 - PMID: 2381442

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105