NIH/3T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

NIH/3T3 (mouse, NIH Swiss, embryo)

Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo
Susceptibility to viruses: herpes simplex * murine leukemia virus * murine sarcoma virus * N-tropic oncornaviruses C * vaccinia * vesicular stomatitis
PROPERTIES: contact inhibition; transformation; transfection; cell biology

Available in the following LABORATORY:

Dip. Scienze Biomediche e Biotecnologie, Universita' degli Studi (BGEBS, Brescia)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 8% DMSO; 37C, 5% CO2

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

NIH/3T3 (mouse, NIH Swiss, embryo)

Monoclonal continuous culture, grown as monolayer
fibroblast morphology
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo
Susceptibility to viruses: herpes simplex * murine leukemia virus * murine sarcoma virus * N-tropic oncornaviruses C * vaccinia * vesicular stomatitis
PROPERTIES: contact inhibition; transformation; transfection; cell biology

Available in the following LABORATORY:

Consorzio Mario Negri SUD, Centro di Ricerche Biomediche e Farmacologiche (CHIBC, S.Maria Imbaro (CH))
DMEM (with 4.5 g/l glucose) + 10% Calf serum; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3 Passage: 133 , mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

NIH/3T3 (mouse, NIH Swiss, embryo)

Available in the following LABORATORY:

Dip. Fisiologia e Biochimica Generali, Universita' degli Studi (MIUBH, Milano)
DMEM + 10% NBCS; Freezing medium: DMEM + 20% NBCS + 10% Glycerol; 37C, 5% CO2, split 20-50 , mycoplasma negative, DAPI and broth-agar

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

NIH/3T3 (mouse, NIH Swiss, embryo)

Available in the following LABORATORY:

Universita' degli Studi (NAUBP, Napoli)
DMEM + 10% FBS; Freezing medium: DMEM + 10% DMSO; 37C, 5% CO2, split 1-10

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

NIH/3T3 (mouse, NIH Swiss, embryo)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
DMEM + 10% FBS; Freezing medium: DMEM + 32% FBS + 8% DMSO; 37C, 5% CO2, split 1-4

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

NIH/3T3 (mouse, NIH Swiss, embryo)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105