SPECIES: mouse, 129
TUMOR: carcinoma, embryonal
DSMZ catalog code: ACC 112
Morphology: adherent cells with undifferentiated morphology growing in clumps
Description: mouse embryonal carcinoma initiated from a testicular teratocarcinoma of a strain 129 mouse confirmed as mouse with IEF of AST, MDH, NP, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: obtained from ATCC (CRL 1720), Rockville, Maryland, USA
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS presoak flasks with 0.1% gelatin at 4C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 70 x 106 cells/80 cm 2;
doubling time of ca. 24 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid karyotype with 6% tetraploidy; 38(37-40)<2n>; 1-2 centric fusion markers present) * isoenzymes (confirmed as mouse with IEF of MDH, NP, PEP B) * reverse transcriptase (reverse transcriptase negative (RDDP assay))
Availability in cell line catalogues: ATCC: CRL 1720, ECACC: 85061803, ICLC: ATL99006
Bibliographic references:
- Cell 1978;15:393-403 - PMID: 214238
- Cell 1980;21:347 - PMID: 6250719
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
- Proc Natl Acad Sci U S A 1973;70:3899 - PMID: 4521215
SPECIES: mouse, 129/SV
TISSUE/ORGAN: embryo
TUMOR: teratocarcinoma
ECACC catalog code: 85061803
Description: Mouse 129 embryo teratocarcinoma
Viruses: See Properties
Depositor: Dr E Robertson, Department of Genetics, University of Cambridge., UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10-15% Fetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS. Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin EDTA; 5% CO2, 37C. Upon resuscitation cells shed a lot of debris, leave for 2 days before subculture. Cells will attach with or without the use of collagen.
Passages: 13;
Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1720, DSMZ: ACC 112, ICLC: ATL99006
Bibliographic references:
- Cell 1978;15:393-403 - PMID: 214238
- Cell 1980;21:347 - PMID: 6250719
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
- Proc Natl Acad Sci U S A 1973;70:3899 - PMID: 4521215
Monoclonal continuous culture, grown as monolayer
undifferentiated morphology
SPECIES: mouse, 129/SV (male, embryo)
TISSUE/ORGAN: testis
TUMOR: teratocarcinoma
tumorigenic in syngeneic animals
PROPERTIES: pluripotent; differentiation
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro (GEICV, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-10 , mycoplasma negative
Growth on 0.1% gelatin-coated dishes. Can be induced to differentiate into parietal or visceral endoderm by retinoic acid LTR blocked, permissive upon differentiation. Obtained from Dr. M.I. Sherman, Dept. Cell Biology, H La Roche Research Center, Nutley, N.J. 07110.
Availability in cell line catalogues: ATCC: CRL 1720, ECACC: 85061803, DSMZ: ACC 112, ICLC: ATL99006
Bibliographic references:
- Cell 1978;15:393-403 - PMID: 214238
- Cell 1980;21:347 - PMID: 6250719
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
- Proc Natl Acad Sci U S A 1973;70:3899 - PMID: 4521215
ICLC catalog code: ATL99006
Continuous culture, grown as monolayer
undifferentiated morphology
SPECIES: mouse, 129
TISSUE/ORGAN: testis
TUMOR: teratocarcinoma
DEPOSITOR: Dr S. Astigiano, Transgenic Unit, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The cells undergo very limited differentiation under normal culture conditions, but can be induced to differentiate into a) parietal endoderm in the presence of retinoic acid (RA) and dibutyryl cyclic AMP; b) visceral endoderm when cultured in suspension as aggregates and treated with RA. Differentiation markers are: tissue type plasminogen activator (tPA) for parietal endoderm cells; alpha fetoprotein (AFP) and urokinase plasminogen activator (uPA) for visceral endoderm cells. In addition, both cell types lose the stage specific embryonic antigen-1 (SSEA-1), and synthesize large amounts of type IV collagen, laminin and fibronectin.
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (4.5 g/L glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x10^4; 37C, 5% CO2 Passage: 6 , mycoplasma negative, HOECHST and PCR
passage 6 after in vivo passage; split every two days; leave the cells with trypsin for one or two minutes at room temperature; the flasks must be presoaked with 0.1% gelatin for 10 minutes
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Availability in cell line catalogues: ECACC: 85061803, DSMZ: ACC 112
Bibliographic references:
- Cell 1978;15:393-403 - PMID: 214238
- Proc Natl Acad Sci USA 1973;70:3899-3903 - PMID: 4521215
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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