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SPECIES: mouse, SIM
ECACC catalog code: 85011404
Description: Mouse SIM embryo teratocarcinoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Mutation and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) + STO feeder layer. Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C. NB: freeze in 10% FBS.
A mutant derived from PSA-1 by exposure to 20uM N-Methyl, N-Nitrosoguanidine for 2 hours, also HPRT-.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc Natl Acad Sci U S A 1977;74:5564-5568 - PMID: 271982
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.