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SPECIES: mouse, BALB/c
ECACC catalog code: 90030803
Susceptibility to viruses: herpes simplex * vesic. stomatitis (Indiana)
Description: Mouse BALB/c embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and transformation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS). Split confluent cultures 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.1% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 40, hypotetraploid
Derived from a 14-17 day old BALB/c mouse embryo. The cells are tumorigenic, are insensitive to contact inhibition and are susceptible to transformation with SV40 virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 164
- J Cell Physiol 1968;72:141-148 - PMID: 4301006
- Science 1968;162:1024-1026 - PMID: 4301647
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.