NIH-3T3

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

NIH-3T3 (mouse, NIH Swiss, embryo)

SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: embryo

DSMZ catalog code: ACC 59
Morphology: fibroblast, adherent monolayer
Susceptibility to viruses: herpes simplex * murine leukemia virus * murine sarcoma virus * N-tropic oncornaviruses C * vaccinia * vesicular stomatitis
Description: Swiss mouse embryo contact-inhibited NIH Swiss mouse embryo; used in DNA transfection assays confirmed as mouse with IEF of AST, MDH, PEP B
Viruses: ELISA: reverse transcriptase negative
Depositor: Dr. H. Hauser, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM (4.5 g/L glucose) + 10% FBS (or NCS) do not allow culture to become completely confluent; split culture at 1:10 every 4 to 6 days using trypsin/EDTA; seed out at ca. 1 x 104 cells/cm 2 at 37C with 5-10% CO2 cell harvest of about 8 x 104 cells/cm 2; doubling time of ca. 20 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.0 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, MHD, PEP B)

Availability in cell line catalogues: ATCC: CRL 1658

Bibliographic references:

Cell 1979;16:347-356 - PMID: 222457
Cell 1979;16:63-75 - PMID: 84715
J Virol 1969;4:549-553 - PMID: 4311790

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105