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SPECIES: mouse, NIH Swiss
ECACC catalog code: 93061524
Susceptibility to viruses: herpes simplex * murine leukemia virus * murine sarcoma virus * N-tropic oncornaviruses C * vaccinia * vesicular stomatitis
Description: Mouse Swiss NIH embryo contact inhibited
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: DNA transfection studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Calf Serum (CS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.
Established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation.
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1658, ICLC: AL07001
- Cell 1979;16:347-356 - PMID: 222457
- Cell 1979;16:63-75 - PMID: 84715
- J Virol 1969;4:549-553 - PMID: 4311790
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.