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ECACC catalog code: 92111707
TRANSFORMED BY: SV40
Description: Mouse, SV40 Polyoma transformed NIH/3T3 cells
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Suitable host for transfection studies, polyoma virus replication studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA, 5% CO2; 37C.
MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of SV 40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1709
- Mol Cell Biol 1984;4:2406-2416 - PMID: 6096696
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.