MDBK

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

MDBK (bovine, kidney)

SPECIES: bovine
TISSUE/ORGAN: kidney

DSMZ catalog code: ACC 174
Morphology: adherent, epithelial-like cells
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis
Description: bovine kidney cells cells were derived from a kidney of a normal adult steer in 1957; cells are BVD-free (bovine diarrhea virus) confirmed as bovine with IEF of AST, G6PD, NP
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institut, BFA, Insel Riems, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
95% Dulbecco's MEM + 5% FBS optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence; seed out 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of about 5-10 x 106 cells/80 cm 2; doubling time of ca. 35 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: isoenzymes (confirmed as bovine with IEF of AST, G6PD, NP)

Availability in cell line catalogues: ATCC: CRL 6071, IZSBS: BS CL63, ECACC: 90050801

Bibliographic references:

Proc Soc Exp Biol Med 1958;98:574