MDBK's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MDBK (bovine, kidney)

SPECIES: bovine
TISSUE/ORGAN: kidney

ECACC catalog code: 90050801
Morphology: Epithelial-like
Description: Bovine kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Bos virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS) (BVDV negative) or 10% Horse Serum (HS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 120; Karyotype: Modal No. 51; hypodiploid
Derived from the kidney of an apparently normal, adult Bos taurus. These cells are BVDV-free. Also known as MDBK (NBL-1).
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 22, IZSBS: BS CL63, DSMZ: ACC 174

Bibliographic references:

J Natl Cancer Inst 1986;76:87-93 - PMID: 3455746
Proc Soc Exp Biol Med 1958;98:574

MDBK (bovine, kidney)

IZSBS catalog code: BS CL63
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: bovine
TISSUE/ORGAN: kidney
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 123 , mycoplasma negative, culture

Availability in cell line catalogues: ATCC: CRL 6071, DSMZ: ACC 174, ECACC: 90050801

MDBK (bovine, kidney)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: bovine
TISSUE/ORGAN: kidney
Susceptibility to viruses: alpha viruses * bovine diarrhea virus * infectious bovine rhinotracheitis * parainfluenza 3 * vesicular stomatitis

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: CRL 6071, IZSBS: BS CL63, DSMZ: ACC 174, ECACC: 90050801

Bibliographic references:

Proc Soc Exp Biol Med 1958;98:574

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105