HuP-T3

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).

HuP-T3 (human, Japanese, pancreas, adenocarcinoma)

ICLC catalog code: HTL96006
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Japanese (male, 66 years old) Karyology: modal chromosome number 37
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: CEA production

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:4-1:10 using trypsin/EDTA(10-15 min); seed at 2-4x104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, NP)

Availability in cell line catalogues: ECACC: 93121055, DSMZ: ACC 259

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

See homonymous cell lines in other collections and catalogues.

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105