HuP-T3's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HuP-T3 (human, Japanese, pancreas, carcinoma)

SPECIES: human, Japanese
TISSUE/ORGAN: pancreas
TUMOR: carcinoma

DSMZ catalog code: ACC 259
Morphology: epitheloid cells growing adherently in monolayers and clusters
Description: human pancreas carcinoma established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984 confirmed as human with IEF of AST, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. S. Saito, Toyama Medical and Pharmaceutical University, Toyama, Japan
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% MEM (with Earle's salts) + 10% FBS + 1% non-essential amino acids + 1% sodium pyruvate split confluent culture 1:10 once a week using trypsin/EDTA; seed out about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 about 14 x 106 cells/175 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 1.4 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (flat-moded hypodiploid karyotype with 13% polyploidy; 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3 mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12); gene amplification) * cytogenetics 2 (suggested by large HSR; ch17 nullisomy) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (100%), desmin-, endothel-, GFAP-, neurofilament-, vimentin-) * isoenzymes (confirmed as human with IEF of AST, NP) * reverse transcriptase (reverse transcriptase not detected)

Availability in cell line catalogues: ECACC: 93121055, ICLC: HTL96006

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

HuP-T3 (human, Japanese, pancreas, adenocarcinoma)

SPECIES: human, Japanese
TISSUE/ORGAN: pancreas
TUMOR: adenocarcinoma

ECACC catalog code: 93121055
Morphology: Epithelial
Description: Human pancreatic adenocarcinoma
Viruses: See Properties
Depositor: Dr S Saito, Toyama Medical and Pharmaceutical University, Toyama, JAPAN (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Products: Carcinoembryonic antigen (CEA)

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1%Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: Modal No 37
Derived from an ascites sample taken from a 66 year old Japanese male with a poorly differentiated pancreatic adenocarcinoma.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: DSMZ: ACC 259, ICLC: HTL96006

Bibliographic references:

Int J Pancreatol 1993;13(1):31-41 - PMID: 8454916

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105