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SPECIES: human, Caucasian
ECACC catalog code: 87070202
Morphology: Flat Epithelial - like
Description: Human Caucasian osteosarcoma, TE85
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus, transformation and biochemical studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 46, hyperdiploid
Derived from a 13 year old female caucasian. Cells are sensitive to both virus and chemical transformation and exhibit a flat morphology, low saturation density and low plating efficiency in soft agar.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1543, IZSBS: BS TCL 69, ICLC: HTL04003
- Cancer 1971;27:397-402
- Int J Cancer 1975;15:23-29 - PMID: 165148
- Int J Cancer 1975;16:840 - PMID: 171229
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.