HOS's homonymous lines

Version 4.0.201701

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HOS (human, Caucasian, bone, osteosarcoma)

ICLC catalog code: HTL04003
Continuous culture, grown as monolayer
fibroblast-like and epithelial-like morphology
SPECIES: human, Caucasian (female, 13 year old)
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: Prof. A. Gasperi-Campani, Sperimental Pathology Dept, University of Bologna, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split sub-confluent (70-80%) cultures 1:10-1:12 twice for week using trypsin/EDTA; 37°C, 5% CO2 Passage: 48 , mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)

Availability in cell line catalogues: IZSBS: BS TCL 69, ECACC: 87070202

Bibliographic references:

Cancer 1971;27:397-402 - PMID: 5100401
Int J Cancer 1975;15:23-29 - PMID: 165148
Int J Cancer 1975;16:840-849 - PMID: 23195

HOS (human, Caucasian, bone, osteosarcoma)

IZSBS catalog code: BS TCL 69
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 13 years old) Karyology: unknown
TISSUE/ORGAN: bone
TUMOR: osteosarcoma
DEPOSITOR: obtained from ATCC
PROPERTIES: viral and chemical transformation

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 27 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human wih NP, G6PD, AST, LD)

Availability in cell line catalogues: ATCC: CRL 1543, ECACC: 87070202, ICLC: HTL04003

Bibliographic references:

Int J Cancer 1975;15:23-29 - PMID: 165148
Int J Cancer 1975;16:840 - PMID: 171229

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105