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SPECIES: mouse, 129/SV
ECACC catalog code: 85061803
Description: Mouse 129 embryo teratocarcinoma
Viruses: See Properties
Depositor: Dr E Robertson, Department of Genetics, University of Cambridge., UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10-15% Fetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS. Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin EDTA; 5% CO2, 37C. Upon resuscitation cells shed a lot of debris, leave for 2 days before subculture. Cells will attach with or without the use of collagen.
Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1720, DSMZ: ACC 112, ICLC: ATL99006
- Cell 1978;15:393-403 - PMID: 214238
- Cell 1980;21:347 - PMID: 6250719
- Exp Cell Res 1980;126:407-416 - PMID: 6153988
- Proc Natl Acad Sci U S A 1973;70:3899 - PMID: 4521215
See homonymous cell lines in other collections and catalogues.
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.