SPECIES: monkey, African green
TISSUE/ORGAN: kidney
ECACC catalog code: 87032605
Morphology: Fibroblast
Susceptibility to viruses: arbovirus * eastern equine encephalitis * herpes simplex * poliovirus 1 * SV40 * western equine encephalitis
Description: Monkey African Green kidney fibroblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Transformation and virus studies
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Karyotype: 2n = 60, pseudodiploid
Used in RSV transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African Green monkey kidney. Cells grow rapidly and form monolayers of fibroblast like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 70
Bibliographic references:
- Proc Natl Acad Sci U S A 1964;53:53
- Virology 1965;27:453 - PMID: 4285109
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Susceptibility to viruses: arbovirus * eastern equine encephalitis * herpes simplex * poliovirus 1 * SV40 * western equine encephalitis
PROPERTIES: virology; transformation
Available in the following LABORATORY:
-
Universita' degli Studi (PRUMB, Parma)
MEM + 10% FBS + 2mM L-Glutamine + non-essential amino acids; Freezing medium: MEM + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 40
Availability in cell line catalogues: ATCC: CCL 70, ECACC: 87032605
Bibliographic references:
- Proc Natl Acad Sci U S A 1964;53:53
- Virology 1965;27:453 - PMID: 4285109
Continuous culture, grown as monolayer
fibroblast morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Susceptibility to viruses: arbovirus * eastern equine encephalitis * herpes simplex * poliovirus 1 * SV40 * western equine encephalitis
PROPERTIES: virology; transformation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 70, ECACC: 87032605
Bibliographic references:
- Proc Natl Acad Sci U S A 1964;53:53
- Virology 1965;27:453 - PMID: 4285109
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
|
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
|
