3T6's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

3T6 (mouse, Swiss albino, fibroblast)

SPECIES: mouse, Swiss albino
TISSUE/ORGAN: fibroblast

DSMZ catalog code: ACC 202
Morphology: fibroblasts growing adherently as monolayer
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia * vesic. stomatitis (Indiana)
Description: Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1963; cells were described to secrete collagen and hyaluronic acid (= ATCC CCL 96) confirmed as mouse with IEF of AST, G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Dulbecco's MEM + 10-15% FBS split confluent culture every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm 2 at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2; doubling time of ca. 15 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine flat-moded hypertriploid-hypotetraploid karyotype; 67(64-73)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, G6PD, MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 96, IZSBS: BS CL 83

Bibliographic references:

J Cell Biol 1963;17:299-313
Virology 1965;27:179-185 - PMID: 4284655

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105