COLO 800's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

COLO 800 (human, subcutaneous nodule, melanoma)

ICLC catalog code: HTL97005
Continuous culture, grown as monolayer
fibroblast. Cells display remarkable blebbing morphology
SPECIES: human (male, 14 years old)
TISSUE/ORGAN: subcutaneous nodule
TUMOR: melanoma
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR

Validated by: isoenzymes (confirmed as human with MD, NP)

Availability in cell line catalogues: ECACC: 93051123

Bibliographic references:

Cancer Genet Cytogenet 1993;68:1002

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105