SPECIES: human
TISSUE/ORGAN: liver
ECACC catalog code: 88021102
Morphology: Epithelial
Susceptibility to viruses: adenovirus 3 * influenza * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
Description: Human liver, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus, biochemical and replication studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 260;
Karyotype: Modal No 70
One of the first cell lines to be derived from non-malignant tissue. Used in virology, biochemistry and transplantation related to malignancy. Cells tend to pile up in cultures with high population density. This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 13, IZSBS: BS CL14
Bibliographic references:
- J Exp Med 1961;113:95
- Natl Cancer Inst Monogr 1962;7:249
- Proc Soc Exp Biol Med 1954;87:440
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: liver
Susceptibility to viruses: adenovirus 3 * influenza * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
PROPERTIES: virology; biochemistry; tumorigenicity; replication; transplantation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 13, IZSBS: BS CL14, ECACC: 88021102
Bibliographic references:
- J Exp Med 1961;113:95
- Natl Cancer Inst Monogr 1962;7:249
- Proc Soc Exp Biol Med 1954;87:440
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: liver
Susceptibility to viruses: adenovirus 3 * influenza * poliovirus 1 * poliovirus 2 * poliovirus 3 * vesic. stomatitis (Indiana)
PROPERTIES: virology; biochemistry; tumorigenicity; replication; transplantation
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 13, IZSBS: BS CL14, ECACC: 88021102
Bibliographic references:
- J Exp Med 1961;113:95
- Natl Cancer Inst Monogr 1962;7:249
- Proc Soc Exp Biol Med 1954;87:440
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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