SPECIES: rat
TISSUE/ORGAN: neural
TUMOR: glial tumor
ECACC catalog code: 92090409
Morphology: Fibroblast-like
Description: Rat glial tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: S-100 protein
Available in the following LABORATORY:
-
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 42
Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the neural tissue of vertebrates. The synthesis of S-100 is dependent on the level of confluence.
Research council deposit: No;
Price Code: B;
Availability in cell line catalogues: IZSBS: BS TCL5, ICLC: ATL95007
ICLC catalog code: ATL95007
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TUMOR: glial tumor
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: S-100 protein production (depending on the level of confluence). HLA: B27.
Available in the following LABORATORY:
-
IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 106 cells/cm2. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with AST, MD, NP)
Availability in cell line catalogues: IZSBS: BS TCL5, ECACC: 85040101, ECACC: 92090409
Bibliographic references:
- Fed Proc 1968;27:720
- Science 1968;161:370 - PMID: 4873531
IZSBS catalog code: BS TCL5
Monoclonal continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TUMOR: glial tumor
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: S-100 protein prod.; biochemistry; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 107, ECACC: 85040101, ICLC: ATL95007, ECACC: 92090409
Monoclonal continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: rat
TUMOR: glial tumor
Susceptibility to viruses: herpes simplex * vaccinia * vesic. stomatitis (Indiana)
PROPERTIES: S-100 protein prod.; biochemistry; virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 107, IZSBS: BS TCL5, ECACC: 85040101, ICLC: ATL95007, ECACC: 92090409
Bibliographic references:
- Fed Proc 1968;27:720
- Science 1968;161:370 - PMID: 4873531
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
|
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
|
