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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

C6 (rat, neural, glial tumor)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Karyotype: 2n = 42
  Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the brain and also in brain tumours from both human and in other animals. The cell line is capable of performing this organ specific function over a period of a year and is capable of producing elevated levels of glyceryl phosphate dehydrogenase activity when stimulated by glucocorticoides. The synthesis of S-100 is dependent on the level of confluence and increases ten fold as cells grow to confluence.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 107, IZSBS: BS TCL5, ICLC: ATL95007

Bibliographic references:

• Fed Proc 1968;27:720
  
• Science 1968;161:370 - PMID: 4873531
  

C6 (rat, neural, glial tumor)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 1-3x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
  Karyotype: 2n = 42
  Derived from a rat glial tumour induced by N-nitrosomethylurea. The cells produce S-100 protein which is found in the brain and also in brain tumours from both human and in other animals. The cell line is capable of performing this organ specific function over a period of a year and is capable of producing elevated levels of glyceryl phosphate dehydrogenase activity when stimulated by glucocorticoides. The synthesis of S-100 is dependent on the level of confluence and increases ten fold as cells grow to confluence.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 107, IZSBS: BS TCL5, ICLC: ATL95007

Bibliographic references:

• Fed Proc 1968;27:720
  
• Science 1968;161:370 - PMID: 4873531
  

C6 (rat, glial tumor)

Available in the following LABORATORY:

• IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
  Ham's F12 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 106 cells/cm2. 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
  Validated by: isoenzymes (confirmed as rat with AST, MD, NP)
  

Availability in cell line catalogues: IZSBS: BS TCL5, ECACC: 85040101, ECACC: 92090409

Bibliographic references:

• Fed Proc 1968;27:720
• Science 1968;161:370 - PMID: 4873531

C6 (rat, glial tumor)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  Ham's F10 + 2.5% FBS + 15% HS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 41 , mycoplasma negative, culture
  Cloned from a rat glial tumor induced by N-nitrosomethylurea. Glyceryl phosphate deydrogenase activity when stimulated by glucocorticoids
  

Availability in cell line catalogues: ATCC: CCL 107, ECACC: 85040101, ICLC: ATL95007, ECACC: 92090409