BC3H1's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

BC3H1 (mouse, brain, tumor)

SPECIES: mouse
TISSUE/ORGAN: brain
TUMOR: tumor

DSMZ catalog code: ACC 171
Morphology: adherent, flat cells forming a confluent monolayer
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: mouse brain tumor established from a mouse tumor induced by nitrosoethylurea with properties characteristic of muscle; cells were described to synthesize adenylate and creatine phosphokinases confirmed as mouse with IEF of AST, PEP B
Viruses: ELISA. reverse transcriptase positive
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% Dulbecco's MEM + 10-20 % FBS split confluent culture 1:5 every week or 1:10 every two weeks (cells grow slowly) using trypsin/EDTA at 37C with 5% CO2 about 1-2 x 106 cells/80 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, PEP B)

Availability in cell line catalogues: ATCC: CRL 1443, ECACC: 86093001, IZSBS: TCL 145

Bibliographic references:

J Biol Chem 1977;252:2143-2153 - PMID: 845167
J Cell Biol 1974;61:398-413 - PMID: 4363958

BC3H1 (mouse, brain, brain tumor)

SPECIES: mouse
TISSUE/ORGAN: brain
TUMOR: brain tumor

ECACC catalog code: 86093001
Morphology: Fibroblast - 2 distinct morphologies (see comments).
Description: Mouse brain tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Acetylcholine Applications: Acetylcholine receptor studies.

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea and possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1443, DSMZ: ACC 171, IZSBS: TCL 145

Bibliographic references:

J Biol Chem 1977;252:2143-2153 - PMID: 845167
J Cell Biol 1974;61:398-413 - PMID: 4363958

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105