WISH's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

WISH (human, amnion)

SPECIES: human
TISSUE/ORGAN: amnion

ECACC catalog code: 88102403
Morphology: Epithelial
Susceptibility to viruses: adenovirus 3 * measles * poliovirus 1 * poliovirus 2 * vesic. stomatitis (Indiana) * vesicular stomatitis
Description: Human amnion, HeLa characteristics
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: VSV (Indiana), Ad 3, Poliovirus, measles virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C.
Passages: 181; Karyotype: 2n = 46
From human amnion tissue. Contaminated with HeLa cells. Have been used in virus studies; susceptible to VSV (Indiana), adenovirus 3 and poliovirus. Useful in differentiating virulent and avirulent measles virus. HeLa marker chromosomes and type A G6PD This cell line was found to have a HeLa profile by DNA fingerprinting.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 25, IZSBS: BS CL92

Bibliographic references:

Am J Dis Child 1962;103:460
Exp Cell Res 1961;23:14
Nature 1976;259:211-213 - PMID: 1250349

WISH (human, amnion)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: amnion
Susceptibility to viruses: adenovirus 3 * measles * poliovirus 1 * poliovirus 2 * vesic. stomatitis (Indiana) * vesicular stomatitis
PROPERTIES: interferon assay; virology

Available in the following LABORATORY:

Istituto di Virologia, Universita' degli Studi (RMUVI, Roma)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: MEM + 10% FBS + 10% Glycerol; 37C, 5% CO2 Passage: 184 , mycoplasma negative
Validated by: cytotoxic ab dye exclusion test * immunofluorescence

Availability in cell line catalogues: ATCC: CCL 25, IZSBS: BS CL92, ECACC: 88102403

Bibliographic references:

Am J Dis Child 1962;103:460
Exp Cell Res 1961;23:14
Nature 1976;259:211-213 - PMID: 1250349

WISH (human, amnion)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human
TISSUE/ORGAN: amnion
Susceptibility to viruses: adenovirus 3 * measles * poliovirus 1 * poliovirus 2 * vesic. stomatitis (Indiana) * vesicular stomatitis
Cell line available under some conditions.
PROPERTIES: interferon assay; virology

Available in the following LABORATORY:

Istituto Fisiologia Generale, Universita' degli Studi (SIUCC, Siena)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2, split 1-3 Passage: 194 , mycoplasma negative

Availability in cell line catalogues: ATCC: CCL 25, IZSBS: BS CL92, ECACC: 88102403

Bibliographic references:

Am J Dis Child 1962;103:460
Exp Cell Res 1961;23:14
Nature 1976;259:211-213 - PMID: 1250349

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105