U-937's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

Monoclonal continuous culture, grown as suspension
histomonocytoid morphology
SPECIES: human, Caucasian (male, 37 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: lymphoma, histiocytic
Susceptibility to viruses: HIV-1 * HIV-2
PROPERTIES: IL-1 prod.; specialized product secr.; tumor markers; Fc receptor; monocytic differentiation; differentiation; antitumour testing; cell biology; virology; tumorigenicity; c-myc mRNA expression; C3b receptor

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-8 , mycoplasma negative, DAPI and HOECHST
Validated by: isoenzymes

Availability in cell line catalogues: ATCC: CRL 1593, DSMZ: ACC 5, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, lymphoma, histiocytic)

SPECIES: human
TUMOR: lymphoma, histiocytic

DSMZ catalog code: ACC 5
Morphology: round to polygonal, single cells in suspension
Susceptibility to viruses: HIV-1 * HIV-2
Description: human histiocytic lymphoma established from the pleural effusion of a 37-year-old man with generalized diffuse histiocytic lymphoma in 1974; cells were described to express markers and properties of monocytes; patented cell line; cells express mRNA for c-myc confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. K. Nilsson, University Hospital, Uppsala, Sweden
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 saturation density at about 2.0 x 106 cells/ml; doubling time of about 30-40 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x10 6 cells/ampoule; negative in DAPI, microbiological culture, PCR assays;
Validated by: cytogenetics 1 (hypotriploid (flat-moded) karyotype; 63(58-69)<3n>XXY, -2, -5, -6, +7, -9, -10, -11, -12, -12, -20, -21, +3mar, der(1)t(1;12)(p33;q13), der(5)t(1;5)(p11;q31), der(9)t(9;?)(p22;?), del(11)(q22), i(11q), der(16)t(16;?)(q24;?),) * cytogenetics 2 (der(19)t(?10;19)(q12;p13.3); expression of monocytic features may be linked to del(11q); 5q33 breakpoint near to that recurrent in histiocytic lymphoma) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (negative for HTLV-I/II, HIV-1)

Availability in cell line catalogues: ATCC: CRL 1593, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

ICLC catalog code: HTL94002
Continuous culture, grown as suspension
histomonocytoid morphology
SPECIES: human, Caucasian (male, 37 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: lymphoma, histiocytic
Susceptibility to viruses: HIV-1 * HIV-2
DEPOSITOR: Obtained from ATCC. Established by C. Sundrom and K. Nilsson in 1974.
Cell line available for distribution. The cell line must not be exploited commercially
PROPERTIES: IL-1 production; specialized product secretion; tumor markers; Fc receptors; antitumour testing; virology; tumorigenicity; expression of markers and properties of monocytes, expression of mRNA for c-myc.

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split 1:5-1:20, maintain cultures between 2 and 9x105 cells/ml. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X; vWA: 14,15,16,17; FGA: 22,25; TH01: 6,9.3; D18S51: 13,14; D21S11: 27,29; D8S1179: 12,13) * isoenzymes (confirmed as human with AST, NP, PepB)

Availability in cell line catalogues: DSMZ: ACC 5

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

Continuous culture, grown as suspension
histomonocytoid morphology
SPECIES: human, Caucasian (male, 37 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: lymphoma, histiocytic
Susceptibility to viruses: HIV-1 * HIV-2
PROPERTIES: IL-1 prod.; specialized product secr.; tumor markers; Fc receptor; monocytic differentiation; differentiation; antitumour testing; cell biology; virology; tumorigenicity; c-myc mRNA expression; C3b receptor

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISB, Milano)
DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-4
Split twice a week.

Availability in cell line catalogues: ATCC: CRL 1593, DSMZ: ACC 5, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 7% CO2, split 1-5
Obtained from Dr. R. Mertelsmann Sloan Kettering New York

Availability in cell line catalogues: ATCC: CRL 1593, DSMZ: ACC 5, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

Available in the following LABORATORY:

ENEA, CRE-Casaccia (RMEBA, Roma)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-9 , mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1593, DSMZ: ACC 5, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

U-937 (human, Caucasian, pleural effusion, lymphoma, histiocytic)

Available in the following LABORATORY:

Istituto Superiore di Sanit� (RMSVI, Roma)
RPMI 1640 + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-8

Availability in cell line catalogues: ATCC: CRL 1593, DSMZ: ACC 5, ICLC: HTL94002

Bibliographic references:

Int J Cancer 1976;17:565-577 - PMID: 178611
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1980;125:463-465 - PMID: 7381208
Nature 1979;279:328-331 - PMID: 450085

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105