SUP-T1's homonymous lines

Version 4.0.201701

Home - Cell Index - Search - Web Sites
Species - Tissues - Tumors - Pathologies - Tr. Agents
Collections - Laboratories - Catalogues

For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SUP-T1 (human, Caucasian, lymphoma, T cell)

SPECIES: human, Caucasian
TUMOR: lymphoma, T cell

DSMZ catalog code: ACC 140
Morphology: single, round cells in suspension
Description: human T cell lymphoma established from the pleural effusion of an 8-year-old boy with T-lymphoblastic lymphoma in relapse confirmed as human with IEF of AST, MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. M. Gramatzki, University Hospital Erlangen, Erlangen, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 2-3 days; after thawing the viability may drop to less than 50%, but the cells recover during the following 4-5 days; seed out at ca. 0.5-1.0 x 10 6 cells/ml at 37C with 5% CO2 maximal density of 1.5-2.0 x 106 cells/ml; doubling time of ca. 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at 5 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid with 2% polyploidy; 85(83-87)<4n>XXYY, inv(2)(p22q11), del(4)(q31q35), del(6)(q25), del(7)(q34), t(9;?)(q34;?), inv(14)(q11q32)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD8+ CD13- CD19-) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase not detected; negative for HTLV-I/II, HIV-1)

Availability in cell line catalogues: ATCC: CRL 1942, ECACC: 95013123, ICLC: HTL96007

Bibliographic references:

Blood 1986;67:650-656 - PMID: 3004618
Cancer Res 1984;44:5657 - PMID: 6437672
Cell 1985;43:705 - PMID: 3935328
Cell 1986;50:107
Science 1984;226:1445 - PMID: 6438800
Science 1986;232:1123 - PMID: 3010463

SUP-T1 (human, Caucasian, lymphoma, lymphoblastic, T cell)

ICLC catalog code: HTL96007
Continuous culture, grown as suspension
single cells, round morphology
SPECIES: human, Caucasian (male, 8 years old)
TUMOR: lymphoma, lymphoblastic, T cell
DEPOSITOR: Obtained from ECACC
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells have been used for the growth of HIV-1. HLA: DR-

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Maintain cultures between 3-9x105 cells/ml; split 1:5-1:10. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with MD, MPI)

Availability in cell line catalogues: ECACC: 95013123, DSMZ: ACC 140

Bibliographic references:

Blood 1986;67:650-656 - PMID: 3004618
Blood 1988;71:395-402 - PMID: 2962650
Cancer Res 1984;44:5657 - PMID: 6437672
Cell 1985;43:705-713 - PMID: 3935328
Cell 1987;50:107 - PMID: 3036364
Science 1984;226:1445 - PMID: 6438800
Science 1986;234:1123 - PMID: 3010463

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105