Monoclonal continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity
Available in the following LABORATORY:
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Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-12.5 , mycoplasma negative, DAPI and HOECHST
Isolated from metastasis to bone marrow
Validated by: isoenzymes
Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;7:410
- Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013
ICLC catalog code: HTL96019
Continuous culture, grown as monolayer
neuroblast-like and epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TUMOR: neuroblastoma
tumorigenic in nude mice
DEPOSITOR: Dr P. Montaldo, G. Gaslini Institute, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: receptor cell line in cell-mediated cytotoxicity
Available in the following LABORATORY:
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IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4x104 cells/cm2. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, G6PD)
Availability in cell line catalogues: ECACC: 86012802
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;7:410
- Proc Natl Acad Sci USA 1978;75:3965-3969 - PMID: 279013
Continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;7:410
- Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013
Monoclonal continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;7:410
- Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013
Continuous culture, grown as monolayer
neuroblast-like or epithelial-like morphology
SPECIES: human, Caucasian (female, 4 years old) Karyology: modal number 47
TISSUE/ORGAN: bone marrow
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; receptor; chemotherapeutic agents; nucleic acids; neural tissue; cytotoxicity
Available in the following LABORATORY:
-
Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Availability in cell line catalogues: ATCC: HTB 11, ECACC: 86012802, ICLC: HTL96019
Bibliographic references:
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;7:410
- Proc Natl Acad Sci U S A 1978;75:3965-3969 - PMID: 279013
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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