Monoclonal continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue
Available in the following LABORATORY:
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Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 10% FBS + 1% non-essential amino acids; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-6 , mycoplasma negative, DAPI and HOECHST
Isolated from metastasis to supra-orbital area
Validated by: isoenzymes
Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203
Bibliographic references:
- Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;8:410
SPECIES: human, Caucasian
TUMOR: neuroblastoma
DSMZ catalog code: ACC 203
Morphology: adherent, fibroblast-like cells growing as monolayers
Description: human neuroblastoma established from the supraorbital metastasis of a neuroblastoma of a 14-year-old girl in 1971 (see ATCC HTB 10); although described in the original paper as neuroblastoma, SK-N-MC is now widely regarded as having originated from the morphologically similar Askin's tumor related to Ewing's sarcoma; FLI1-EWS gene fusion, the molecular pathological change in Ewing's sarcoma and Askin's tumor, was shown in SK-N-MC by Dunn et al. (Cancer Genet. Cytogenet. 76: 19-22, 1994) confirmed as human with IEF of AST, MDH
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions
Available in the following LABORATORY:
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German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 1-4 x 106 cells/80 cm 2 ; cells may grow slowly initially at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2;
doubling time of ca. 48 hours;
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule;
negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (pseudodiploid karyotype with 6% polyploidy; 46(40-47)<2n>XX, +8, -16, t(1;4) (p36;p13), t(2;3)(q24;q29), der(3)t(3;3)(p13;q22), del(10)(q25), t(11;22)(q24;q12) t(15;22)(q21;q12), add(17)(p12), add(21)(p12); dmin in 9% of cells;) * cytogenetics 2 (resembles published karyotype; carries masked 11;22 translocation recurrent in Ewing's and Askin's tumor.) * DNA fingerprinting (unique DNA profile with (gtg)5 multilocus probe) * immunological analysis (cytokeratin+ (5-10%), desmin-, endothel-, GFAP-, neurofilament-, vimentin+ (100%)) * isoenzymes (confirmed as human with IEF of AST, MDH) * reverse transcriptase (reverse transcriptase negative)
Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302
Bibliographic references:
- Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;8:410
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203
Bibliographic references:
- Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;8:410
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue
Available in the following LABORATORY:
-
Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203
Bibliographic references:
- Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;8:410
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 14 years old)
TUMOR: neuroblastoma
tumorigenic in nude mice
PROPERTIES: biochemical markers; catecholamine prod.; receptor; chemotherapeutic agents; neural tissue
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: HTB 10, ECACC: 90022302, DSMZ: ACC 203
Bibliographic references:
- Cancer Genet Cytogenet 1994;76:19-22 - PMID: 8076344
- Cancer Res 1973;33:2643-2652 - PMID: 4748425
- In Vitro 1973;8:410
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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