SiHa's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

SiHa (human, Japanese, cervix, carcinoma, squamous cell)

ICLC catalog code: HTL98017
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Japanese (female, 55 years old)
TISSUE/ORGAN: cervix
TUMOR: carcinoma, squamous cell
DEPOSITOR: Dr. I. Bongarzone, Istituto Nazionale Tumori, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: MEM + 50% FBS + 10% DMSO; spilt confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with MD, Pep B)

Bibliographic references:

Am J Pathol 1985;119:361-366 - PMID: 2990217
Cancer Res 1997;57:2112-2115 - PMID: 9187105
Cancer Res 1997;57:5528-5533 - PMID: 9407963
J Virol 1987;61:962-971 - PMID: 3029430
Proc Natl Acad Sci USA 1991;88:5523-5527 - PMID: 1648218
Proc Soc Exp Biol Med 1970;135:543-545 - PMID: 5529598
Virology 1985;145:313-318 - PMID: 2992153

SiHa (human, Mongoloid, cervix, carcinoma, squamous cell)

Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Mongoloid (female)
TISSUE/ORGAN: cervix
TUMOR: carcinoma, squamous cell
tumorigenic in nude mice

Available in the following LABORATORY:

Istituto Nazionale per lo Studio e la Cura dei Tumori (MIISA, Milano)
MEM + 10% FBS + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-10

Availability in cell line catalogues: ATCC: HTB 35, ICLC: HTL98017

Bibliographic references:

Proc Soc Exp Biol Med 1970;135:543-545 - PMID: 5529598

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105