MOLT-4's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
Cell line available under some conditions.
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Dipartimento Oncologia e Neuroscienze, Universita'degli Studi (CHUIM, Chieti)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

SPECIES: human
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic

ECACC catalog code: 85011413
Morphology: Suspension
Susceptibility to viruses: alpha viruses * measles
Description: Human acute T lymphoblastic leukaemia
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Karyotype: Modal No. 95; hypertetraploid
A suspension culture derived from the peripheral blood of a 19 year old male with acute lymphoblastic leukaemia in relapse. A stable T-cell leukaemia that forms rosettes with sheep erythrocytes.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, peripheral blood, leukemia, T cell)

ICLC catalog code: HTL99025
Continuous culture, grown as suspension
round cells, single or in clumps, lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
DEPOSITOR: Dr G. Canti, Pharmacology Dept, Milan, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: G->A mutation at codon 248 of the p53; CD1+ CD2+ CD3+ CD4+ CD5+ CD6+ CD7+ CD19- EBV-; high level of terminal deoxynucleotidyl transferase

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 20% FBS + 2mM L-Glutamine; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 5-10 x 10^5 cells/ml; split 1:3-1:6; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231
J Natl Cancer Inst 1980;65;715:718 - PMID: 6932523
Proc Natl Acad Sci USA 1990;87:7555-7559 - PMID: 16992228

MOLT-4 (human, peripheral blood, leukemia)

IZSBS catalog code: BS TCL 85
Continuous culture, grown as suspension
Lymphoblast-like morphology
SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia
DEPOSITOR: obtained from ATCC
Distribution is subjected to authorization by the assignee.
Patent strain

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, MPI)

Availability in cell line catalogues: ATCC: CRL-1582, ICLC: HTL99025, ECACC: 85011413, DSMZ: ACC 362

Bibliographic references:

J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

HeLa markers. At the moment the availability of this cell line is not sure

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Monoclonal continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Centro Trapianto Midollo Osseo e Divisione Ematologia (PSHTM, Pesaro)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + Antibiotics + 1mM Na pyruvate; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-2

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Available in the following LABORATORY:

Istituto Superiore di Sanità (RMSVI, Roma)
RPMI 1640 + 10% FBS; Freezing medium: 90% FBS + 10% DMSO; 37C, 5% CO2, split 1-7

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Dip.Medicina Sperimentale e Scienze Biochimiche, Universita' degli Studi (RMUFM, Roma)
RPMI 1640 + 15% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Validated by: T cell markers

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

MOLT-4 (human, lymphocyte, T, leukemia, acute lymphoblastic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human (male, 19 years old)
TISSUE/ORGAN: lymphocyte, T
TUMOR: leukemia, acute lymphoblastic
tumorigenic in nude mice
Susceptibility to viruses: alpha viruses * measles
PROPERTIES: antigen presentation; biochemical markers; antitumour testing; biochemistry; immunology; tumor cytotoxicity; tumorigenicity; differentiation; target line; cytotoxicity; virology

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CRL 1582, ICLC: HTL99025, ECACC: 85011413, IZSBS: BS TCL 85, DSMZ: ACC 362

Bibliographic references:

Int J Immunopharmacol 1988;10:907-911 - PMID: 3145923
J Immunol 1982;129:2504-2510 - PMID: 6982930
J Natl Cancer Inst 1972;49:891-895 - PMID: 4567231

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105