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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

KG-1 (human, Caucasian, bone marrow, leukemia, acute myeloid)

Available in the following LABORATORY:

• Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
  IMDM + 20% FBS; Freezing medium: Culture medium + 5% DMSO; 37C, 5% CO2, split 1-4 , mycoplasma negative, DAPI and HOECHST
  Validated by: isoenzymes
  

Availability in cell line catalogues: ATCC: CCL 246, ECACC: 86111306, DSMZ: ACC 14

Bibliographic references:

• Blood 1980;56:344-350 - PMID: 6996765
• Blood 1983;62:709-721 - PMID: 6192859
• Science 1978;200:1153-1154 - PMID: 306682

KG-1 (human, bone marrow, leukemia, acute myeloid)

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% RPMI 1640 + 10% FBS maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days; seed out at ca. 1 x 106 cells/ml at 37C with 5% CO2 maximum density at 1.0-1.5 x 106 cells/ml; doubling time of ca. 38 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays;
  Validated by: cytogenetics 1 (hypodiploid with 5% polyploidy; 45(42-47)<2n>X, -Y, -7, -12, +2m, dup(7)(q12q33), der(8)t(8;?)(p12;?), der(11)t(1;11)(q11;p11), der(17)t(17;?)(p13;?); does not correspond clearly with original karyotype) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+) * isoenzymes (confirmed as human with IEF of AST, MDH, NP) * reverse transcriptase (reverse transcriptase negative)
  

Availability in cell line catalogues: ATCC: CCL 246, ECACC: 86111306

Bibliographic references:

• Blood 1980;56:344-350 - PMID: 6996765
  
• Blood 1983;62:709-721 - PMID: 6192859
  
• Science 1978;200:1153-1154 - PMID: 306682
  

KG-1 (human, Caucasian, bone marrow, leukemia, acute myeloid)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  IDMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
  Karyotype: 2n = 46, diploid
  Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.
  Research council deposit: No; Price Code: B;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 246, DSMZ: ACC 14

Bibliographic references:

• Blood 1980;56:344-350 - PMID: 6996765
  
• Blood 1983;62:709-721 - PMID: 6192859
  
• Science 1978;200:1153-1154 - PMID: 306682