HL-60's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning

Available in the following LABORATORY:

Istituto Nazionale Riposo e Cura Anziani (INRCA) (ANIIR, Ancona)
RPMI 1640 + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-6

Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

Available in the following LABORATORY:

Clinica Pediatrica, Ospedale S.Orsola-Malpighi (BOHIC, Bologna)
RPMI 1640 + 20% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-6 , mycoplasma negative, DAPI and HOECHST
Split every 3-4 days
Validated by: B cell markers

Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

HL-60 (human, peripheral blood, leukemia, acute myeloid)

SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute myeloid

DSMZ catalog code: ACC 3
Morphology: round, single cells in suspension
Susceptibility to viruses: HIV-1 * HTLV-1
Description: human acute myeloid leukemia established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described to be responsive to DMSO, phorbol ester TPA and other reagents and to carry amplified c-myc gene; present cells are apparently tetraploid derivates of hypodiploid original where c-myc was amplified in dmin (instead of hsr) confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. E. Porfiri, The Royal Free Hospital, Department of Haematology, London, UK
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:5 every 1-2 days; seed out at about 1 x 106 cells/ml at 37C with 5% CO2 maximal density of 1.5-2.0 x 106 cells/ml; doubling time of about 25 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (hypotetraploid karyotype without clear mode, 1.5% polyploidy; 82(78-88)<4n>XX, -X, -X, -2, -3, -4, -5, -8, -9, -10, -14, -16, -17, -17, +3m, der(6)t(6;?)(q25;?)/dup(6)(q23;qter)x2, del(9)(p22), del(11)(q22/23), der(16)t(16;17)) * cytogenetics 2 ((q22/23;q21-22)x2; high frequency of subclonal ch 1 rearrangements) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (CD3- CD13+ CD14- CD15+ CD19- CD33+ HLA-DR-) * isoenzymes (confirmed as human with IEF of MDH, NP) * reverse transcriptase (not detected (RDDP assay))

Availability in cell line catalogues: ATCC: CCL 240, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

ICLC catalog code: HTL95010
Continuous culture, grown as suspension
lymphoblast-like morphology
SPECIES: human, Caucasian (female, 36 years old) Karyology: pseudodiploid
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, promyelocytic
tumorigenic in nude mice
Susceptibility to viruses: HIV-1 * HTLV-1
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: phagocytosis; antitumour testing; differentiation; pharmacodynamics; cloning

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10; maintain cultures between 5-9x105 cells/ml; 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
After treatment with DMSO the cells differentiate along the granulocytic line (after 4 days in 1,25% DMSO, 72% of the cells become CD11b positive).
Validated by: isoenzymes (confirmed as human with MD, MPI, NP)

Availability in cell line catalogues: DSMZ: ACC 3

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci USA 1978;75:2458-2462 - PMID: 276884

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 Passage: 510
Obtained from Dr. Mertelsmann Sloan Kettering New York

Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

Available in the following LABORATORY:

Dip.Medicina Sperimentale e Scienze Biochimiche, Universita' degli Studi (RMUFM, Roma)
RPMI 1640 + 15% FBS + Antibiotics + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative
Validated by: immunofluorescence

Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

HL-60 (human, Caucasian, peripheral blood, leukemia, promyelocytic)

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)

Availability in cell line catalogues: ATCC: CCL 240, DSMZ: ACC 3, ICLC: HTL95010

Bibliographic references:

Blood 1979;54:713-733 - PMID: 288488
Blood 1988;71:242-247 - PMID: 3422031
Nature 1977;270:347-349 - PMID: 271272
Proc Natl Acad Sci U S A 1978;75:2458-2462 - PMID: 276884

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105