Hep G2's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
Cell line available under some conditions.
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Istituto Patologia Generale, Universita' degli Studi (GEUCC, Genova)
MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-5 , mycoplasma negative, HOECHST
Aneuploidies, partial trisomies, translocations, pericentric inversions, chrom. 1 rearrangements
Validated by: monooxygenase expression and induction

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

ICLC catalog code: HTL95005
Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 15 years old)
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
DEPOSITOR: Prof. M. Ferro, DIMES, General Pathology, University of Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells are reported to produce: alpha-fetoprotein, albumin, alpha2-macroglobulin, alpha1-antitrypsin, transferrin, alpha1-antichimotrypsin, haptoglobin, ceruloplasmin, plasminogen, C3,C4, C3 act., fibrinogen, alpha1-acid glycop., alpha2-HS glycoprotein, beta lipoprotein and retinol binding protein; xenobiotic metabolism, drug metabolism assays, toxicity screening, hepatocyte studies

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2-3 x 10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
Validated by: identification confirmed by Short Tandem Repeat (STR) profile (Amelogenin: X,Y; vWA: 17; FGA: 22,25; TH01: 9; D18S51: 13,14; D21S11: 29,31; D8S1179: 15,16) * isoenzymes (confirmed as human with MD, MPI)

Availability in cell line catalogues: IZSBS: BS TCL79, ECACC: 85011430

Bibliographic references:

Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

IZSBS catalog code: BS TCL79
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
MEM (EBSS) + 10% FBS + 1mM Na pyruvate; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 94 , mycoplasma negative, culture
Kariotype: 2n=55 in 50 cells. Patent strain, distribution is subjected to authorization by the assignee. Tumor: hepatoblastoma.

Availability in cell line catalogues: ATCC: HB 8065, ECACC: 85011430, ICLC: HTL95005

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Pharmacia Upjhon (MIECC, Nerviano (MI))
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; Freezing medium: DMEM + 20% FBS + 10% Glycerol; 37C, 5% CO2, split 2-3 , mycoplasma negative
Epithelial-like morphology

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Istituto di Scienze Farmacologiche, Universita' degli Studi (MIUFC, Milano)
MEM + 10% FBS + Antibiotics + 1% non-essential amino acids + 20mM Tricine + 1mM Na pyruvate; Freezing medium: Culture medium + 20% FBS + 10% DMSO; 37C, 5% CO2, split 1-4
Validated by: biochemical assays * cellular assays

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Universita' degli Studi (PGUPG, Perugia)
RPMI 1640 + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

Russian Academy of Sciences (SPBIC, St. Petersburg)
mycoplasma negative

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

Hep G2 (human, Caucasian, liver, carcinoma, hepatocellular)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (male, 15 years old) Karyology: modal number 55
TISSUE/ORGAN: liver
TUMOR: carcinoma, hepatocellular
non tumorigenic
PROPERTIES: biochemical markers; hormone prod.; lipid prod.; tumor markers; protein secr.; serum complement components prod.; biochemistry; enzymology; factor sensibility; metabolism; receptor; replication; toxicity screening; tumorigenicity; differentiation; cytotoxicity; xenobiotic metabolism; lipoprotein; virology

Available in the following LABORATORY:

USL 8, Divisione Gastroenterologia, Ospedale Molinette (TOHEP, Torino)
MEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-3
Susceptibility to viral transfection. 2n=54. Rearranged chromosome 6.

Availability in cell line catalogues: ATCC: HB 8065, IZSBS: BS TCL79, ECACC: 85011430, ICLC: HTL95005

Bibliographic references:

In Vitro Cell Dev Biol 1987;23:349-354 - PMID: 3034851
J Lipid Res 1986;27:858 - PMID: 3021884
Nature 1979;282:615-616 - PMID: 233137
Science 1980;209:497-499 - PMID: 6248960

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105