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For information, get in touch with: Dott.ssa Paola Visconti Email: paola.visconti@hsanmartino.it		P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932. DOI: doi:10.1093/nar/gkn730; PMID: 18927105

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• Dip. Scienze Biomediche e Biotecnologie, Universita' degli Studi (BGEBS, Brescia)
  MEM + 10% FBS + 1mM Na pyruvate; Freezing medium: MEM + 10% FBS + 8% DMSO; 37C, 5% CO2 , mycoplasma negative, HOECHST
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
• J Exp Med 1953;97:695
• J Gen Virol 1983;64:825 - PMID: 6834007
• J Virol 1978;28:444 - PMID: 214572
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
  90% MEM (with Earle's salts) + 10% FBS + 2 mM L-glutamine + non-essential amino acids (cells also grow well in 90-95% RPMI 1640 + 5-10% FBS) split confluent culture 1:4 to 1:6 every 3-5 days using trypsin/EDTA; cells reach confluence quickly; seed out at ca. 1-2 x 106 cells/80 cm 2 at 37C with 5% CO2 cell harvest of ca. 8-15 x 106 cells/80 cm 2; doubling time of ca. 48 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
  Validated by: DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological analysis (cytokeratin+ desmin- endothel- GFAP- neurofilament- vimentin+) * isoenzymes (confirmed as human with IEF of GPDH, MDH, NP) * reverse transcriptase (reverse trancriptase not detected)
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
  
• J Exp Med 1953;97:695
  
• J Gen Virol 1983;64:825 - PMID: 6834007
  
• J Virol 1978;28:444 - PMID: 214572
  
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173
  

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
  Passages: 9; Karyotype: 2n = 46, aneuploid
  Derived from a cervical carcinoma from a 31 year old Negro. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2.
  Research council deposit: No; Price Code: A;
  Validated by: isoenzymes (species confirmation)
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
  
• J Exp Med 1953;97:695
  
• J Gen Virol 1983;64:825 - PMID: 6834007
  
• J Virol 1978;28:444 - PMID: 214572
  
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173
  

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
  MEM (EBSS) + 10% FBS; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2 , mycoplasma negative, culture
  

Availability in cell line catalogues: ATCC: CCL 2, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• Boehringer Mannheim Italia S.p.A. (MIEOS, Monza (MI))
  MEM (EBSS) + 10% Human serum + 1% non-essential amino acids; Freezing medium: Culture medium + 15% Human serum + 5% Glycerol; 37C, 5% CO2 , mycoplasma negative
  Validated by: isoenzymes
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
• J Exp Med 1953;97:695
• J Gen Virol 1983;64:825 - PMID: 6834007
• J Virol 1978;28:444 - PMID: 214572
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• Russian Academy of Medical Sciences (MWIIW, Moscow)
  mycoplasma negative
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
• J Exp Med 1953;97:695
• J Gen Virol 1983;64:825 - PMID: 6834007
• J Virol 1978;28:444 - PMID: 214572
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173

HeLa (human, Black, cervix, carcinoma, epitheloid)

Available in the following LABORATORY:

• ENEA, CRE-Casaccia (RMEBA, Roma)
  DMEM + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split1-9 , mycoplasma negative
  

Availability in cell line catalogues: ATCC: CCL 2, IZSBS: BS TCL20, ECACC: 93021013, DSMZ: ACC 57, ICLC: HTL95023

Bibliographic references:

• Cancer Res 1952;12:264
• J Exp Med 1953;97:695
• J Gen Virol 1983;64:825 - PMID: 6834007
• J Virol 1978;28:444 - PMID: 214572
• Obstet Gynecol 1971;38:945-949 - PMID: 4942173