SPECIES: rat, AxC
TISSUE/ORGAN: liver
TUMOR: hepatoma
ECACC catalog code: 85061112
Morphology: Epithelial
Endogenous viruses: contains type C
Description: Rat liver hepatoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Enzymatic and virus studies: Type C virus
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 15;
From H-35 Reuber hepatoma. Tyrosine aminotransferase positive. Synthesizes several serum proteins including albumin, transferrin, prothrombin. The presence of type C virus has been shown and can readily be verified by electron microscopy.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1600, IZSBS: BS TCL 127
Bibliographic references:
- Biochimie 1974;56:1603-1611 - PMID: 4157008
- J Natl Cancer Inst 1961;26:891
- Natl Cancer Inst Monogr 1964;13:229
IZSBS catalog code: BS TCL 127
Continuous culture, grown as monolayer
Epithelial-like morphology
SPECIES: rat, AxC (male)
TISSUE/ORGAN: liver
TUMOR: hepatoma
Endogenous viruses: contains type C
DEPOSITOR: obtained from ATCC
PROPERTIES: used for studies on enzyme regulation aminoacid transport protein syhthesis and degradation bilirubin conjugation collagen synthesis; producing several proteins
Available in the following LABORATORY:
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Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSBS, Brescia)
DMEM (4.5 g/L glucose) + 10% FBS; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 7 , mycoplasma negative, culture
Validated by: isoenzymes (confirmed as rat with NP, G6PD, MD, MPI, PEPB, AST, LD)
Availability in cell line catalogues: ATCC: CRL-1600, ECACC: 85061112
Bibliographic references:
- J Natl Cancer Inst 1961;26:891
- Natl Cancer Inst Monogr 1964;13:229
- Science 1972;178:1098
Monoclonal continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: rat, AxC (male)
TISSUE/ORGAN: liver
TUMOR: hepatoma
tumorigenic in syngeneic animals
Endogenous viruses: contains type C
PROPERTIES: monooxygenases expression; albumin prod.; transferrin prod.; prothrombin prod.; enzymology; factor sensibility; toxicity screening; somatic cell hybridization; gene expression; xenobiotic metabolism; virology
Available in the following LABORATORY:
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Universita' degli Studi (PGUPG, Perugia)
Swim's S77 medium + 20% HS + 5% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 10% Glycerol; 37C, 5% CO2
Tyrosine aminotransferase positive
Availability in cell line catalogues: ATCC: CRL 1600, ECACC: 85061112, IZSBS: BS TCL 127
Bibliographic references:
- Biochimie 1974;56:1603-1611 - PMID: 4157008
- J Natl Cancer Inst 1961;26:891
- Natl Cancer Inst Monogr 1964;13:229
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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