A-204's homonymous lines

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For information, get in touch with: Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

P. Romano, A. Manniello et al. Nucl Acids Res 2009 37:D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105

A-204 (human, muscle, rhabdomyosarcoma)

ICLC catalog code: HTL99002
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 1 year old)
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma
DEPOSITOR: Prof. A. Colombatti, Centro di Riferimento Oncologico, Aviano, Italy
Cell line available for distribution. For non-commercial investigative use only

Available in the following LABORATORY:

IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 2 -4x10^4 cells/cm2; 37C, 5% CO2 , mycoplasma negative, HOECHST and PCR
The cells are difficult to detach
Validated by: HLA-DP beta (confirmed as human by PCR)

Availability in cell line catalogues: DSMZ: ACC 250

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-204 (human, muscle, rhabdomyosarcoma, alveolar)

Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human (female, 1 year old)
TISSUE/ORGAN: muscle
TUMOR: rhabdomyosarcoma, alveolar
tumorigenic in nude mice
PROPERTIES: adhesion; biochemistry; virology

Available in the following LABORATORY:

Russian Academy of Medical Sciences (MWIIW, Moscow)

Availability in cell line catalogues: ATCC: HTB 82, DSMZ: ACC 250, ICLC: HTL99002

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-204 (human, muscle, rhabdomyosarcoma, alveolar)

Available in the following LABORATORY:

Centro di Riferimento Oncologico (PNIOS, Aviano)

Availability in cell line catalogues: ATCC: HTB 82, DSMZ: ACC 250, ICLC: HTL99002

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

A-204 (human, muscle, rhabdomyosarcoma, alveolar)

Available in the following LABORATORY:

Universita' La Sapienza (RMUPG, Roma)
RPMI 1640 + 10% FBS; Freezing medium: RPMI 1640 + 20% FBS + 10% DMSO; 37C, 5% CO2 , mycoplasma negative

Availability in cell line catalogues: ATCC: HTB 82, DSMZ: ACC 250, ICLC: HTL99002

Bibliographic references:

J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758

For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it

If you want to cite CLDB and HyperCLDB, please use the following reference:

P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730; PMID: 18927105