SPECIES: fish - minnow
TISSUE/ORGAN: skin
ECACC catalog code: 88102401
Morphology: Epithelial-like
Susceptibility to viruses: catfish tumor virus * ECHO-11 * FV-1 * FV-3 * * infectious pancreatic necrosis * vesic. stomatitis (Indiana)
Description: Fish Fat Head minnow
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff's frog viruses
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2; 34C. Dislodge cells by agitation and scraping. Cells multiply over a wide range of temperatures 0-36C, with maximum growth at about 34C.
Passages: 80;
Karyotype: Modal No 51
Derived from tissue posterior to the anus of normal adult minnows. Capable of growth over wide temperature range from 4C-36C with maximum growth at 34C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 42, IZSBS: BS CL45
Bibliographic references:
- Ann N Y Acad Sci 1965;126:555 - PMID: 5220177
- In Vitro 1980;16:168 - PMID: 6767657
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: fish - minnow
TISSUE/ORGAN: skin
Susceptibility to viruses: catfish tumor virus * ECHO-11 * FV-1 * FV-3 * * infectious pancreatic necrosis * vesic. stomatitis (Indiana)
PROPERTIES: virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 42, IZSBS: BS CL45, ECACC: 88102401
Bibliographic references:
- Ann N Y Acad Sci 1965;126:555 - PMID: 5220177
- In Vitro 1980;16:168 - PMID: 6767657
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: fish - minnow
TISSUE/ORGAN: skin
Susceptibility to viruses: catfish tumor virus * ECHO-11 * FV-1 * FV-3 * * infectious pancreatic necrosis * vesic. stomatitis (Indiana)
PROPERTIES: virology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 42, IZSBS: BS CL45, ECACC: 88102401
Bibliographic references:
- Ann N Y Acad Sci 1965;126:555 - PMID: 5220177
- In Vitro 1980;16:168 - PMID: 6767657
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
Email: paola.visconti@hsanmartino.it
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If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.
DOI: doi:10.1093/nar/gkn730;
PMID: 18927105
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