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SPECIES: human, Black
TUMOR: lymphoma, Burkitt
ECACC catalog code: 85011437
Endogenous viruses: contains Epstein-Barr
Description: Human Negroid Burkitt's lymphoma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals.
Karyotype: 2n = 46, diploid
Derived from 16-yr old male Negro. Positive for EBNA, carries EBV markers, Complement Receptors, surface bound immunoglobulin and surface markers for Fc fragment of IgG. The cells should be handled under laboratory containment level 2.
Research council deposit: No;
Price Code: B;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 213, DSMZ: ACC 78, ICLC: HTL95024, IZSBS: BS TCL 104
- Cancer Res 1968;28:1300-1310 - PMID: 4174339
- Clin Exp Immunol 1976;25:367-368 - PMID: 963908
- Int J Cancer 1977;19:334-337
- Tissue Antigens 1978;11:96-112 - PMID: 77067
See homonymous cell lines in other collections and catalogues.
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.