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ICLC catalog code: HTL08004
Continuous culture, grown as monolayer
Transfected by: Transfected with wt EGFR Back To Transfection WebPage
DEPOSITOR: Dr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy,
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium 45% + 50% FCS + 5% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Handle as Biosafety Level 2 containment
Validated by: Multiplex PCR (tested again human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
- Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7727-31 - PMID: 8052651
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.