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ICLC catalog code: HTL06007
Continuous culture, grown as monolayer
SPECIES: mouse, NIH Swiss
TISSUE/ORGAN: kidney, embryonic
DEPOSITOR: Dr M. Dominici, Oncology Dept, University of Modena e Reggio Emilia, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: Cell line can be used to produce retroviral vector
Available in the following LABORATORY:
- IRCCS Ospedale Policlinico San Martino (ICLC, Genova)
DMEM (4.5g/l glucose) + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 5% DMSO; split cultures 1:5-1:10 using trypsin/EDTA, 37C, 5% CO2 Passage: 6, mycoplasma negative, HOECHST and PCR
Handle under Biosafety Level 2 containment
Ecotropic packaging cell line
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
- Blood 1997 Sep 1;90(5):1777-86 - PMID: 9292510
For information, get in touch with:
IRCCS Ospedale Policlinico San Martino
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Dott.ssa Paola Visconti
If you want to cite CLDB and HyperCLDB, please use the following reference:
P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines.
Nucleic Acids Research 2009 37(Database issue):D925-D932.